Cell Analysis Service—Helios (a CyTOF System) Mass Cytometer

Summary

The core laboratory has one Helios mass cytometer for operator-assisted data acquisition. For mass cytometry, cells are labeled with antibodies conjugated to metal isotopes, introduced as individual cells into the Helios where they are ionized and analyzed by a time-of-flight mass spectrometer. This novel approach overcomes the difficulties associated with fluorescence overlap that limits polychromatic flow cytometry and permits the simultaneous measurement of over 40 parameters on single cells. The core facility has established a reagent bank of metal-conjugated antibodies that is comprised of common reagents of interest to investigators that require high-dimensional single cell phenotypic and functional analysis.