Cell Imaging Service—Leica TCS SP8 Confocal Microscope

As cell research advances, so does the need for cell imaging technology. Today's researchers need a continuous flow of dynamic, long-term experiments on cell migration, interactions, and responses to environmental disturbances that cannot be met by conventional microscopes that can only observe stationary cells. Creative Bioarray' Leica TCS SP8 Confocal Microscope-based live cell imaging technology can meet your stringent experimental needs.

Name of the Instrument/Platform

Leica TCS SP8 Confocal Microscope

MANUFACTURER: Leica

Introduction

Analysis of immunofluorescence as well as functional and biochemical kinetic studies can be performed on viable cell samples. The instrument is equipped with a Quad Black live cell chamber allowing for temperature control and allowing for long term observation of viable cell cultures. Intracellular distribution of fluorescence can also be quantified by way of 3D imaging. The instrument has extremely high sensitivity and can differentiate between closely emitting chromophores. In addition, the Leica TCS SP8 features 3 HyDs (hybrid detectors combining the characteristics of a classic PMT with a highly sensitive avalanche photodiode) and 1 PMT which can measure real time response of time-critical sequences due to its fast scan speed. The HyD detectors allow for the use of very low laser power to preserve sensitive samples and allow for repeated scans with minimal fluorescence bleaching. The instrument has a maximum image resolution of 64 Mpixels. Each laser can fire independently and detectors can be reprogrammed multiple times allowing for multi-color analyses. Leica TCS SP8 Specifications 1. Microscope 1) Leica DMi8 (inverted) 2) Live cell chamber (Quad Black) 3) Motorized scanning stage 2. 4 air cooled lasers 1) 405 nm direct modulation laser 2) 488 nm solid state laser 3) 552 nm solid state laser 4) 638 nm solid state laser 3. Objectives 1) 10×/0.40 2) 20×/0.70 3) 40×/1.25-0.75 oil 4) 63×/1.20 water 5) 63×/1.40 oil 6) 63×/1.30 glycerin

Summary

This laser based confocal system permits measurements of fluorescently labeled tissue sections, individual cells, or particles adhered to matrices over a wide selection of visible wavelengths. The instrument is equipped with 4 air cooled lasers allowing for excitation wavelengths of 405, 488, 552 and 638 nm.

Technical Capabilities

The Leica TCS SP8 Confocal Microscope-based cell imaging service offered by Creative Bioarray supports high-resolution fluorescence and brightfield image acquisition, enabling real-time recording of data over hours, days, or weeks. If you need our help, please contact us directly.