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Cell Imaging Service—Olympus IX83 TIRF/Single Molecule Localization Microscope

As cell research advances, so does the need for cell imaging technology. Today's researchers need a continuous flow of dynamic, long-term experiments on cell migration, interactions, and responses to environmental disturbances that cannot be met by conventional microscopes that can only observe stationary cells. Creative Bioarray' Olympus IX83 TIRF/Single Molecule Localization Microscope-based live cell imaging technology can meet your stringent experimental needs.

Name of the Instrument/Platform

Olympus IX83 TIRF/Single Molecule Localization Microscope

Introduction

Microscope Details 1. Custom Olympus IX83 inverted microscope with ASI motorized XY stage with piezo Z insert. 2. Excelitas Turbo multi-spectral LED (wide field) and Lumencor white LED (bright field) 3. 20×/0.8NA oil, 60×/1.5NA oil TIRF, 60×/1.2NA water, and 100×/1.4NA oil objectives 4. DIC capable for 60× and 100× optics. 5. Olympus Real Time Controller for temporally accurate camera and excitation synchronization 6. 4 laser line MITICO TIRF illuminator for independent pentration depths 7. 4 individual laser lines for TIRF: 405 nm (100mW) ; 488 nm (100mW) ; 561 nm (100mW) and 640 nm (140mW) 8. Direct Stochastic Optical Reconstruction Microscopy (dSTORM), Photoactivated Localization Microscopy (PALM) & Highly Inclined Laminar Optical (HILO) sheets imaging possible 9. Hamamatsu Fusion sCMOS camera and Geminini W-View image splitter for 2 color simultaneous live cell acquisitons. 10. Non-photodestructive IR ZDC2 autofocus for extremely fast one shot or continuous autofocus capabilites 11. BiopTechs objective heater for short-term live cell applications 12. Olympus cellSens Dimension image acquisition software

Feature Highlights

Applications

1. Fixed specimens with #1.5H cover slips 2. Live cell specimens (LabTekII #1.5H multi-well or 35 mm (#1.5H) cover bottom) 3. 3D acquisition (Z-Stacks) and Time Lapse imaging 4. Specific applications include live-cell TIRF single molecule detection, HILO, 2D dSTORM or PALM

Technical Capabilities

The Olympus IX83 TIRF/Single Molecule Localization Microscope-based cell imaging service offered by Creative Bioarray supports high-resolution fluorescence and brightfield image acquisition, enabling real-time recording of data over hours, days, or weeks. If you need our help, please contact us directly.

For research use only, not intended for any clinical use.

Online Inquiry

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