Confocal Microscope Imaging Service—Zeiss LSM780-FCS

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. Creative Bioarray, the imaging experts, can provide you with Zeiss LSM780-FCS-based confocal microscopy imaging services.

Name of the Instrument/Platform

Zeiss LSM780-FCS

MANUFACTURER: Zeiss

Introduction

1. Zeiss AxioObserver with 780-Quasar (34-channel spectral, high-sensitivity gallium-arsenide phosphide detectors, GaAsP) confocal module & fluorescence correlation spectroscopy (FCS) 2. Environmental chamber for 37℃, CO₂, & humidity (35mm dishes or chamber slides); Heater box for body of microscope. 3. Definite Focus nosepiece (fast auto-focus to compensate for focus drift during sample heating) 4. NA 0.55 condenser (dry only) with DIC for 20×, 40×, 63× and 100× lenses 5. Objectives: 10×/0.45 PlanApo (dry, no DIC) 20×/0.80 PlanApo (dry, no DIC) 40×/1.30 PlanNeofluar oil, with DIC 40×/1.20 C-Apo water, with DIC, upon request 63×/1.4 PlanApo oil, with DIC 100× PlanApo oil, with DIC, upon request 6. 7 laser lines available: 405 (diode laser), 458, 477, 488, 514 (argon laser) 561 (solid-state) 639 (diode laser) 7. H×P 100W metal-halide illuminator for wide-field fluorescence 8. Fluorescence filter sets (wide-field; non-confocal): #49 for DAPI, #38 for GFP, #43 For Cy3 9. Latest Zen software to control microscope and confocal 10. Zen software on a separate workstation for image analysis. 11. Download Zen LE software available for desktop computers in your lab Analysis with Volocity, Imaris or ImageJ on our analysis workstations Use LOCI BioFormats to import images into ImageJ

Summary

Zeiss AxioObserver with 780-Quasar confocal module

Feature Highlights

Applications

1. Single confocal images or image stacks for 3D viewing 2. FRAP with user-defined photobleach marks 3. CFP/YFP FRET 4. Photoactivation at 405 nm of caged dyes and other molecules 5. Colocalization of different fluorescent signals 6. Speed and versatility 7. Quasar "fingerprint" system separates very closely spaced fluorochromes. Example: DPPIV-GFP (Golgi, green) and Albumin-Alexa 488 (ER-Golgi, red)

Technical Capabilities

Creative Bioarray' Zeiss LSM780-FCS-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.