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Cellular and Molecular Imaging Service—Teneo VolumeScope with SEM

Creative Bioarray has established one of the most complete biological laboratories with extensive, state-of-the-art equipment. We have provided high-quality analysis technical support to many customers from around the world. Customer satisfaction is our mission and our technicians supply cellular and molecular imaging service based on Teneo VolumeScope with SEM.

Name of the Instrument/Platform

Teneo VolumeScope with SEM

Introduction

Teneo VolumeScope is a Scanning Electron Microscope (SEM) with an inbuild ultramicrotome for serial block-face scanning electron microscopy (SBF SEM). The Thermo Scientific VolumeScope with Multi-Energy Deconvolution (MED) is a state-of-the-art SBF SEM that combines physical and optical slicing technologies with 10 nm isotropic 3D datasets of resin embedded biological samples.

Summary

This field-leading 10 nm isotropic resolution is possible through the use of MED-SEM technology, allowing optical sectioning to derive several virtual subsurface layers within each physical slice, thus dramatically improving resolution, particularly in the axial direction. Acquired volumes are typically larger than those collected with Focused Ion Beam SEM (FIB SEM) technology.

Feature Highlights

Filament: Field emission gun (FEG) Beam current range: 1 pA to 400 nA Landing energy range: 20 eV – 30 keV* Accelerating voltage range: 350 eV – 30 keV Imaging modes: High Vacuum, Low Vacuum (10-50 Pa) Magnifications: up to 300.000 x MapsTM software integration for wide-field tissue mapping (Tiling and Stitching). On-stage compact microtome with easy installation for in-situ sectioning. Optimal imaging resolution at low voltages (1.0 nm at 1 keV using the immersion lens) for 2D SEM images. SBF SEM datasets capable of multiple ROI collection during the same job acquisition with 10-nm isotropic resolution.

Applications

The instrument can also be utilized as a stand-alone SEM and can be used to visualize the surface topology of samples. The microscope can be used in both high- and low-vacuum mode. In low-vaccum mode the sample may contain water and no metal coating.

Technical Capabilities

Creative Bioarray has established one of the most complete biological laboratories with extensive, state-of-the-art equipment. We have provided high-quality cell analysis technical support to many customers from around the world. entering into a partnership with you. Please do not hesitate to contact us with any queries.

For research use only, not intended for any clinical use.

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