WELCOME TO CREATIVE BIOARRAY
WELCOME TO CREATIVE BIOARRAY
Creative Bioarray offers analytical technology solutions for the detection of cellular bioenergy based on the XF24-3 Analyzer. Real-time cellular assays help to understand the key functions that drive cell signaling, proliferation, activation, toxicity, and biosynthesis. It is not limited to the analysis of cellular states, but also provides a clear understanding of changes in cell dynamics.
Unlike radiometric assays, the XF24-3 does not require radiolabeled substrates, and unlike measurements of cellular oxygen consumption using the classic Clark electrode, it is possible to make high throughput measurements on 24 samples simultaneously in a microplate format. The XF24-3 uses disposable fluorescent-based optical sensors to measure oxygen consumption rate (OCR), carbon dioxide production rate (COPR), and extracellular acidification rate (ECAR) directly in the culture media of culture microplates. These values inform about the flux through mitochondrial oxidative metabolism (OCR) and glucose oxidation (ECAR). In addition, through built-in injection ports surrounding each of the culture wells in the microplate, it is possible to sequentially deliver up to 4 compounds (for example, fatty acid, glucose, 2,4-dinitrophenol, FCCP, oligomycin) to the cells during the course of an experiment to determine changes in cellular metabolism in response to specific inhibitors or other compounds.
The XF24-3 is a unique instrument that allows quantitation of cellular energetic pathways in cultured cells in real time. The XF24-3 quantifies the activity of the two major energy-yielding pathways in the cell, mitochondrial respiration and glycolysis. The XF24-3 instrument provides a significant advance over existing methodologies to quantitate cellular metabolic pathways.
A XF Islet Capture Microplate is available to assess whole islet bioenergetics in vitro (30-70 islets per well, glucose or oligomycin response studies). There is a possibility to use this kit to assess respiration from tissue biopsies.
The XF Technology: 1. Measures oxygen consumption rate (OCR), extracellular acidification rate (ECAR) and carbon dioxide production rate (COPR) of living cells simultaneously in real time. 2. Completely non-invasive measurement requires no addition of dyes, labels or reporters. 3. Cell types assayed on the XF analyzer: Cell lines, primary cells, isolated mitochondria, small tissue pieces (pancreatic islets, tissue biopsies from animals) 4. Typically requires only 30,000 to 80,000 cells per well in 24-well plates 5. Cells and plates are not affected by XF measurement and may be used for an additional assay. 6. Measurements may be repeated multiple times to measure kinetic responses. 7. No cleaning required. All parts that contact cells, media or drugs are disposable. 8. Measures adherent cells without requiring trypsinization. 9. Up to four test compounds (drugs or substrates) may be added automatically to each well. Measurements are performed before and after each compound is added.
Creative Bioarray offers its customers XF24-3 Analyzer-based cell bioenergetics analysis services. If you are interested, please contact us directly.
For research use only, not intended for any clinical use.
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