Confocal Microscope Imaging Service—Nikon Ti2 Inverted Microscope with Yokogawa W1 and SoRa Module

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. Creative Bioarray, the imaging experts, can provide you with Nikon Ti2 Inverted Microscope with Yokogawa W1 and SoRa Module-based confocal microscopy imaging services.

Name of the Instrument/Platform

Nikon Ti2 Inverted Microscope with Yokogawa W1 and SoRa Module

MANUFACTURER: Nikon

Introduction

XY Resolution to 120 nm 1. Utilizing magnification and microlensing, an approximately 1.4× improvement beyond the optical limit is achieved, which can further be improved by deconvolution to 2×. 2. The field of view of the CSU-W1 is nearly 4 times that of the CSU-X1, making it ideal for large area scanning applications and low magnification imaging of large specimens. This large field of view is a perfect match for large format sensors. 3. CSU-W1 can be configured with a motorized relay lens for magnification switching, allowing users to change magnification to the detector without changing the microscope's objective lens. 4. Super-resolution images are acquired optically, on any sample, without special preparations or computation. This allows image collection to be limited only by the sample's signal-to-noise ratio and the exposure time of the detector. Video-rate or faster super-resolution imaging is possible. 5. Microlensing the emission pinhole at the correct magnification counteracts the mismatch between an on-axis excitation PSF and the effective confocal PSF (product of excitation and emission PSFs) through an emission pinhole that is not infinitely small. By microlensing, individual points' divergence angle onto the pinhole is reduced 2×, mimicking the effect of an infinitely small ideal pinhole, but not compromising signal brightness.

Summary

The microscope base is a fully motorized Nikon Ti2 with Perfect Focus System (PFS, interferometric-based focus maintenance). The system also has a piezo stage with a 200 μm range capable of up to 100 steps/sec, mounted in a fully motorized XY stage. There is also an environmental control chamber for long-term live-cell imaging.

Feature Highlights

A wider field of view and higher image quality than previous models. 1.2× Resolution Improvement vs. Widefield 2. Enhanced Resolution and Confocal Sectioning Simultaneously 3. Normal Confocal Mode and SoRa Mode 4. Ultra-wide Field of View 5. Enhanced Signal-to-Noise Ratio 6. Flexible Configurations

Technical Capabilities

Important Notes

Laser Platform A Yokogawa spinning disc (CSU-W1) is mounted with two dichroics; a quad dichroic accommodating 405, 488, 561, and 647 nm lasers and a dual dichroic accommodating 454 and 514 nm lasers. The spinning disc detector is a Hamamatsu BT Fusion sCMOS (6.5 μm pixel with 93% QE).

Creative Bioarray' Nikon Ti2 Inverted Microscope with Yokogawa W1 and SoRa Module-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.