Confocal Microscope Imaging Service—Zeiss LSM800 GaAsP

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. Creative Bioarray, the imaging experts, can provide you with Zeiss LSM800 GaAsP-based confocal microscopy imaging services.

Name of the Instrument/Platform

Zeiss LSM800 GaAsP

MANUFACTURER: Zeiss

Introduction

1. Specifications: 2. Zeiss A×ioObserver inverted microscope with LSM800 confocal module 3. High-sensitivity GaAsP detectors (more sensitive than the bi-alkali detectors of our LSM700; comparable sensitivity to the GaAsP array on our LSM780) 4. Definite Focus II module to maintain independent specimen focus at multiple positions (original Definite Focus module could only maintain one focus across multiple positions) 5. High-speed tiling with wide-field CCD camera; integrated control for acquiring confocal images defined by regions within tiled wide-field preview 6. Dedicated 37℃ heater/CO₂/humidity for 35mm dishes, chamber slides, or microtiter plates; large enclosure for stable temperature control 7. NA 0.55 condenser with DIC for 10×, 20×, 40×, and 63× lenses 8. Objectives: 1) 5×/0.16 PlanApo (dry, no DIC) 2) 10×/0.45 PlanApo (dry), with DIC 3) 20×/0.80 PlanApo (dry), with DIC 4) 40×/1.30 PlanNeofluar oil, with DIC 5) 40×/1.20 C-Apo water, with DIC, upon request 6) 63×/1.4 PlanApo oil, with DIC 7) 100×/1.4 PlanApo oil, with DIC, upon request 9. 4 laser lines available: 1) 405 (diode laser) 2) 488 (solid-state) 3) 561 (solid-state) 4) 639 (diode laser) 10. Long-life ×-Cite 120LEDBoost illuminator for wide-field fluorescence 11. Latest Zen software to control microscope and confocal 12. Zen LE software available for desktop computers in your lab 1) Analysis with Volocity, Imaris or ImageJ on our analysis workstations 2) Use LOCI BioFormats to import images into ImageJ

Summary

Zeiss AxioObserver with LSM800 confocal module with upgraded GaAsP detectors

Feature Highlights

Applications

1. Scan large specimens (brain & other tissue) using CCD camera and select sub-regions for confocal scanning 2. Single confocal images or image stacks for 3D viewing 3. Live-cell imaging with temperature and CO₂ control 4. Photoactivation at 405 nm of caged dyes and other molecules 5. Colocalization of different fluorescent signals 6. Speed and versatility

Technical Capabilities

Creative Bioarray' Zeiss LSM800 GaAsP-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.